SRP0718
TRKA (G595R), Active
PRECISIO® Kinase, recombinant
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
UNSPSC Code:
12352202
NACRES:
NA.77
recombinant
expressed in baculovirus infected Sf9 cells
assay
70-95% (SDS-PAGE)
form
frozen liquid
specific activity
2.0-3.0 nmol/min·mg
mol wt
~66 kDa
concentration
0.05 μg/μL
accession no.
NM_002529
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... TRKA(4914)
Application
Useful for kinase assay.
Biochem/physiol Actions
Recombinant human TRKA (G595R) (440-end) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. TRKA is a member of the trk proto-oncogene family and encodes a 140kDa, membrane-spanning protein tyrosine kinase that is the functional receptor for nerve growth factor (NGF). NGF elicits the rapid phosphorylation of gp140trk on tyrosine residues leading to increased c-Fos expression, DNA synthesis and morphologic transformation (1). A decreased expression of TRKA on the striatal cholinergic neurons has been observed which may contribute, when it reaches a crucial threshold, to the death of cholinergic neurons observed in Alzheimer disease (2).
Reconstitution
Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
Storage and Stability
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service