recombinant
expressed in baculovirus infected Sf9 cells
assay
70-95% (SDS-PAGE)
form
frozen liquid
mol wt
~82 kDa
concentration
0.1 μg/μL
accession no.
NM_004567
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PFKFB4(5210)
Application
Useful for Western blot.
Biochem/physiol Actions
Recombinant human PFKFB4 (2-end) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. PFKFB4 (also known as 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase-4) regulates the steady-state concentration of fructose-2, 6-bisphosphate, a potent activator of a key regulatory enzyme of glycolysis pathway called phosphofructokinase. Isozymes of PFKFB differ in the regions surrounding the catalytic core, which are important for the differential response to allosteric effectors and hormonal signals in different tissues (1). PFKFB4 is highly expressed in brain, heart, liver, muscle, placenta, adipose tissue, ovary, fallopian tubes, and testis (2).
Reconstitution
Formulated in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
Storage and Stability
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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