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recombinant
expressed in baculovirus infected Sf9 cells
assay
75-95% (SDS-PAGE)
form
frozen liquid
mol wt
~83 kDa
concentration
0.1 μg/μL
accession no.
NM_004566
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PFKFB3(5209)
application
Useful for Western blot.
Biochem/physiol Actions
Recombinant full-length human PFKFB3 was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. PFKFB3 or 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 is a potent activator of a key regulatory enzyme in the glycolysis pathway called phosphofructokinase. Phosphofructokinase regulates the steady-state concentration of fructose-2,6-bisphosphate, a potent activator of key regulatory enzyme of the glycolysis pathway. PFKFB3 is bifunctional, although the phosphatase activity is low due to a mutation in an active site which can be upregulated after phosphorylation by protein kinases (1). PFKFB3 may be responsible for sustaining the high glycolytic flux of rapidly proliferating leukemia cells and may represent a molecular target for chemotherapeutic intervention (2).
Reconstitution
Formulated in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
Storage and Stability
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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