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SRP0570

Sigma-Aldrich

5′-NUCLEOTIDASE/CD73, His-Tag

recombinant, expressed in HEK 293 cells

Synonym(s):

5′- nucleotidase, Ecto-5′-nucleotidase

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50 μG
$480.00

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50 μG
$480.00

About This Item

UNSPSC Code:
12352202
NACRES:
NA.26

$480.00

Check Cart for Availability
Request a Bulk Order

recombinant

expressed in HEK 293 cells

form

frozen liquid

mol wt

58.6 kDa

UniProt accession no.

P21589

shipped in

dry ice

Storage temp.

−70°C

Gene Information

human ... NT5E(4907)

General description

5′-Nucleotidase/CD73 is glycosyl-phosphatidyl inositol (GPI)- anchored cell surface protein. Itis present in various tissues and cells including endothelial cells, epithelialcells, and subsets of B and T cells.[1]

Application

5′-Nucleotidase/CD73 has been used as a standard in recombinanthuman CD73 assay.[2]

Biochem/physiol Actions

5′-Nucleotidase/CD73 actsas a rate-limiting enzyme to produce adenosine. It helps in upholding tissuehomeostasis by converting the adenosine triphosphate (ATP)-triggered immuneactivation to adenosine-mediated immunosuppression.CD73 is a novel immunoinhibitory protein that participates in tumor growthand metastasis. CD73 in combination with conventional therapy and/or otherimmune checkpoint inhibitors has been reported to show therapeutic effectsagainst cancer.[1]

Physical form

Aqueous solution

Pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
7118490326
7118490319

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Amanda de Fraga Dias et al.
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 162, 105823-105823 (2021-03-31)
Glioblastoma (GBM) is the most frequent and aggressive brain tumor in adults and the current treatments only have a modest effect on patient survival. Recent studies show that bozepinib (BZP), a purine derivative, has potential applications in cancer treatment. The
Targeting CD73 to augment cancer immunotherapy
Meejeon Roh, et al.
Current Opinion in Psychiatry, 53, 66-76 (2020)

Questions

  1. What is the volume of the product in the vial? Additionally, what is the composition of the aqueous buffer?

    1 answer

    1. 1.Based on the pack size of 50 µg and the concentration of 0.88 mg/ml on the Certificate of Analysis (COA), the calculated volume is 56.81 µl.

      2.The product (SRP0570) contains an aqueous buffer with the following specifications:

      •Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
      •Purity: ≥90%
      •Specific Activity: ≥ 20 nmol/min/μg
      •Molecular Weight: 58.6 or 59 kDa
      Tag(s): C-terminal His-tag
      •Amino Acids: 27-547
      •Assay Conditions: Assay was conducted in 50 µL assay buffer containing 25 mM Tris, pH 7.4, 5 mM MgCl2, and 100 µM AMP for 12 minutes at 37°C. •Inorganic phosphate produced was measured using Biomol Green reagent.
      •Format: Aqueous buffer solution

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