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Key Documents

SRP0443

Sigma-Aldrich

UCHL3 active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥90% (SDS-PAGE)

Synonym(s):

UCHL3, Ubiquitin Carboxy-terminal Hydrolase L3

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected Sf9 cells

assay

≥90% (SDS-PAGE)

form

aqueous solution

mol wt

27.1 kDa

packaging

pkg of 100 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

2.07 mg/mL

NCBI accession no.

NM_006002

UniProt accession no.

P15374

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... UCHL3(7347)

General description

UCHL3, also known as Ubiquitin Carboxy-terminal Hydrolase L3 (GenBank Accession No. NM_006002) amino acids 2-231 (end) with N-terminal His-tag, MW=27.1 kDa, expressed in E. coli.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
8035300826

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Nam, M.J., et al.
Proteomics, 3, 2108-2115 (2004)
H Wada et al.
Biochemical and biophysical research communications, 251(3), 688-692 (1998-10-29)
NEDD8 is a novel ubiquitin-like protein that has been shown to conjugate to nuclear proteins in a manner analogous to ubiquitination and sentrinization. To identify proteins that are involved in the NEDD8-conjugation and de-conjugation pathway, the yeast two-hybrid system was
Rieko Setsuie et al.
Neurochemistry international, 54(5-6), 314-321 (2009-01-22)
Ubiquitin (Ub) carboxy terminal hydrolase (UCH)-L1 and UCH-L3 are two of the deubiquitinating enzymes expressed in the brain. Both gad mice, which lack UCH-L1 expression and Uchl3 knockout mice exhibit neurodegeneration, although at distinct areas. These phenotypes indicate the importance

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