Human PHF8 (also known as JHDM1F), (GenBank Accession No. NM_001184896), amino acids 2 - 450 with N-terminal FLAG-tag, MW=52 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Physical form
Formulated in 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 ug/mL Flag peptide, 20% Glycerol, and 3 mM DTT.
While reversible histone modifications are linked to an ever-expanding range of biological functions, the demethylases for histone H4 lysine 20 and their potential regulatory roles remain unknown. Here we report that the PHD and Jumonji C (JmjC) domain-containing protein, PHF8
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