SRP0241

EGFR (T790M) Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

• Synonym(s): ERBB1, Epidermal growth factor receptor, HER1, PIG61, Proto-oncogene c-ErbB-1
• UNSPSC Code: 12352200

Properties

• biological source: human
• recombinant: expressed in baculovirus infected insect cells
• assay: ≥70% (SDS-PAGE)
• form: aqueous solution
• mol wt: 88 kDa
• packaging: pkg of 10 μg
• concentration: >0.02 mg/mL
• NCBI accession no.: NM_005228
• UniProt accession no.: P00533
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... EGFR(1956)

Description

General description

Human EGFR T790M (GenBank Accession No. NM_005228), (amino acids 668-1210) with N-terminal GST tag, MW = 88 kDa, expressed in baculovirus expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Unit Definition

One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Tyr substrate per minute at pH 7.4 and 30°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.