SRP0220
DEP1 Active human
recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
Synonym(s):
CD148, DEP-1, Density-enhanced phosphatase 1, PTPRJ, Protein tyrosine phosphatase, receptor type, J
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20 μG
$371.00
About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
biological source
human
recombinant
expressed in E. coli
assay
≥90% (SDS-PAGE)
form
aqueous solution
potency
≥0.114 units/mg
mol wt
58.9 kDa
packaging
pkg of 20 μg
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PTPRJ(5795)
General description
Protein tyrosine phosphatase (density-enhanced phosphatase-1, PTP-eta, CD148), Catalytic domain, (amino acids 1024-1306) (Gene bank accession no. NM_U10886) with N-terminal GST tag, MW=58.9 kDa, expressed in an E. coli expression system.
Application
Useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates.
Unit Definition
One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
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James R Whiteford et al.
Molecular biology of the cell, 22(19), 3609-3624 (2011-08-05)
Syndecan-2 is a heparan sulfate proteoglycan that has a cell adhesion regulatory domain contained within its extracellular core protein. Cell adhesion to the syndecan-2 extracellular domain (S2ED) is β1 integrin dependent; however, syndecan-2 is not an integrin ligand. Here the
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