SRP0188

Pyruvate kinase M2 Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• Synonym(s): M2-PK tumor, OPA-interacting protein 3 (OIP-3), Pyruvate kinase 3 (PK3), Pyruvate kinase, muscle, Thyroid hormone-binding protein 1 (THBP1)
• UNSPSC Code: 12352200

Properties

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥90% (SDS-PAGE)
• form: aqueous solution
• mol wt: 58.9 kDa
• storage condition: avoid repeated freeze/thaw cycles
• concentration: >0.02 mg/mL
• NCBI accession no.: NM_002654
• UniProt accession no.: P14618
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... PKM2(5315)

Description

General description

Human Pyruvate kinase M2 (PKM2) (GenBank Accession No. NM_002654) full length with N-terminal His tag, MW = 58.9 kDa, expressed in an E. coli expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

Pyruvate kinase M2 (PKM2) is an isoform of pyruvate kinase that is expressed in certain differentiated cells and in proliferatively active cells. PKM2 is often overexpressed in cancer cells.

Unit Definition

One unit is defined as the amount of enzyme required to transfer a phosphoryl group from phosphoenolpyruvate (PEP) to 1 pmol of ADP/min at 37°C

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.