SRP0130

EZH1/EED/SUZ12  human

recombinant, expressed in baculovirus infected insect cells

• Synonym(s): ENX-2, EZH1, Enhancer of zeste homolog 1, KMT6B, Lysine N-methyltransferase 6B
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in baculovirus infected insect cells
• assay: ≥80% (SDS-PAGE)
• form: aqueous solution
• mol wt: 51 kDa (EED); 86 kDa (EZH1); 87 kDa (SUZ12)
• packaging: pkg of 50 μg
• concentration: >0.02 mg/mL
• NCBI accession no.: NM_001991 (EZH1); NM_003797 (EED); NM_015355 (SUZ12)
• UniProt accession no.: Q92800
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... EZH1(2145)

Description

General description

Complex of: Human EZH1, GenBank Accession No. NM_001991, amino acids 2-end, with N-terminal His tag, MW = 86 kDa. Human EED, NM_003797, amino acids with N-terminal DDDDK tag (FLAG), MW = 51 kDa. Human SUZ12,. NM_015355, amino acids 2-end, with N-terminal His tag.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Unit Definition

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide and 10% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.