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SRE0022

Sigma-Aldrich

β-Glucuronidase from abalone

Chromatographically purified, lyophilized powder, β-glucuronidase ≥20,000,000 units/g protein

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About This Item

Enzyme Commission number:
3.1.2.31 (BRENDA, IUBMB)
EC Number:
232-606-8
MDL number:
MFCD00130629
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

abalone

form

lyophilized powder

β-glucuronidase activity

≥20,000,000 units/g protein

secondary activity

≤4,000,000 units/g protein sulfatase

composition

protein, 10-40%

storage temp.

−20°C

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Application

β-Glucuronidase from abalone has been used in enzyme treatment to know the effect of osmotic shock, enzymatic incubation, pulsed electric field, and high shear homogenization on the release of water-soluble proteins and carbohydrates from the green alga Ulva lactuca.[1]
Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine.

Biochem/physiol Actions

β-Glucuronidase (GUSB) is a lysosomal enzyme. It degrades glucuronate-containing glycosaminoglycan.[2] β-glucuronidase, obtained from abalone has the ability to hydrolyze samples, that has high analyte concentrations.[3] Deficiency of β-glucuronidase results in mucopolysaccharidosis type VII (MPSVII), which leads to lysosomal storage in the brain.[2]

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).

Physical form

Supplied as a lyophilized powder containing Trizma® and L-arginine

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLBX7788
SLBW2919
SLBT2965
SLBM4618V

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Evaluation of abalone beta-glucuronidase substitution in current urine hydrolysis procedures
Malik W B, et al.
Journal of Analytical Toxicology, 38(3), 171-176 (2014)
Biorefinery of the macroalgae Ulva lactuca: extraction of proteins and carbohydrates by mild disintegration
Postma P R, et al.
Journal of Applied Phycology, 1-13 (2018)
Human beta-glucuronidase: structure, function, and application in enzyme replacement therapy
Naz H, et al.
Rejuvenation Research, 16(5), 352-363 (2013)
P R Postma et al.
Journal of applied phycology, 30(2), 1281-1293 (2018-05-15)
The effect of osmotic shock, enzymatic incubation, pulsed electric field, and high shear homogenization on the release of water-soluble proteins and carbohydrates from the green alga Ulva lactuca was investigated in this screening study. For osmotic shock, both temperature and

Articles

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

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