SML1734

FHZ

≥98% (HPLC)

• Synonym(s): 2-Amino-3′-hydroxy-6′-(2-(2-(2-hydroxyethoxy)ethoxy)ethoxy)spiro[isoindoline-1,9′-xanthen]-3-one
• Empirical Formula (Hill Notation): C₂₆H₂₆N₂O₇
• CAS Number: 1883737-63-0
• Molecular Weight: 478.49
• UNSPSC Code: 12352200

Properties

• assay: ≥98% (HPLC)
• form: powder
• storage condition: protect from light
• color: white to light brown
• solubility: DMSO: 30 mg/mL, clear
• storage temp.: 2-8°C
• SMILES string: OC1=CC(OC(C=C(OCCOCCOCCO)C=C2)=C2C34N(N)C(C5=C4C=CC=C5)=O)=C3C=C1

Description

Biochem/physiol Actions

FHZ is a highly selective, cell-permeable, non-cytotoxic, non-fluorescent 6-triethylene glycol-substituted fluorescein hydrazide that is an efficient fluorescent turn-on probe for dual channel detection of (•OH) and HOCl in live cells and zebrafish embryos. FHZ selectively reacts with hydroxyl (•OH) and HOCl, respectively, to form highly fluorescent FOBA (Ex: 410 nm & Em.: 486 nm; Φ = 0.42; ε410 = 12000/M/cm) and F-TEG (Ex: 490 nm & Em.: 520 nm; Φ = 0.34; ε490 = 7000/M/cm), while exhibiting no reactivity toward H2O2 ¹O2, O2•–, NO, or ONOO–. Cyan and green fluorescence dual-channel detection with two exciting wavelengths (405 nm and 488 nm) and two collection windows (430–490 nm; 510–560 nm) allows time-dependent quantitative detection of cellular •OH and HOCl by FHZ in live cells (50-100 μM; HeLa and RAW 264.7 macrophages) cells and zebrafish embryos (50 μM).

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable