recombinant
expressed in Pichia pastoris
form
lyophilized powder
specific activity
>500 units/mg protein
mol wt
14.4 kDa
storage condition
room temperature (at +4 °C (in Storage Buffer))
storage temp.
2-8°C
General description
Auresine is a portion of the zinc metalloprotease Staphylococcus aureus. The enzyme has glycyl-glycine endopeptidase activity and it specifically cleaves polyglycine crosslinks in the cellular wall of Staphylococcus species, including various strains of methicillin-resistant Staphylococcus aureus (MRSA). Auresine is thus useful for lysis of MRSA.
Application
Auresine can be used for isolation of protoplasts, nucleic acids, proteins, lipids and other components of Gram-positive bacterial cells. Auresine is particularly useful to lyse MRSA.. Thanks to its unique activity in non-physiological conditions (low conductivity and low temperatures), isolated components after auresine treatment, like nucleic acids and proteins, are not prone to degradation by released cellular enzymes.
Unit Definition
One unit will reduce the turbidity (A600) of suspension of S. aureus cells from 0.250 to 0.125 in 10 minutes in 50 mM glycine, pH 8.0 at 25°C in 6.0 mL reaction mixture.
Preparation Note
Auresine in soluble in water (20 mg/ml), yielding a clear solution. The product is active in 100 nM concentration (1.4 ug/mL) in low conductivity buffers (= 2 mS/cm) at temp. 0-40°C.
Storage and Stability
Auresine lyophilized powder should be stored at room temperature. Once dissolved in storage buffer, Auresine is stable at room temperature up to 4 months, at 4°C, -20°C, -80°C at least 2 years. Storage buffer: 20 mM Tris-HCl, pH 7.0, 200 mM NaCl, 10% glycerol.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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