This product is tested for unit of activity per mL, so the volume varies and is lot-dependent. Please refer to the sample Certificate of Analysis for enzyme activity.
SAE0023
Alanine Racemase from Bacillus stearothermophilus
buffered aqueous solution, ≥10 IU/mg solid
Synonym(s):
Alanine Racemase from Bacillus stearothermophilus
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100 UNITS
$425.00
About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
$425.00
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biological source
Bacillus sp. (B. stearothermophilus)
Quality Level
form
buffered aqueous solution
specific activity
≥10 IU/mg solid
mol wt
Mr 78 kDa (2 subunits 39 kDa each)
Storage temp.
−20°C
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Application
Alanine racemase is used to convert L-alanine into D-alanine. Alanine racemase, from Sigma, has been used to isomerize L-[U-14C]alanine to a racemic mixture of L/D- [14C]alanine †.
Biochem/physiol Actions
Alanine racemase is involved in alanine, aspartate and D-alanine metabolism. 3-Fluoro-D-alanine and D-Cycloserine are known to inhibit alanine racemase.† Alanine racemase monomer is composed of two domains, an eight-stranded α/β barrel at the N-terminus and a C-terminal domain. The N-terminus includes residues 1-240, whereas the C-terminal comprises of the β-strand (residues 241-388). One molecule of pyridoxalphosphate (PLP) is present as the cofactor in each subunit.†
Unit Definition
One unit will convert 1.0 μmole of D-alanine to L-alanine per minute at pH 10.5 at 30 °C in a coupled assay system with L-alanine dehydrogenase.
Physical form
Aqueous solution containing 10 mM potassium phosphate
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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What is the concentration and/or volume of this solution?
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What is the amino acid sequence of the Bacillus stearothermophilus alanine racemase that you are selling (SAE0023)?
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SAE0023 is isolated from Bacillus sp. The published sequence can be found here:
https://www.uniprot.org/uniprotkb/P10724/entryHelpful?
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