SAB5200020

Monoclonal Anti-MDC1 antibody produced in mouse

clone P2B11, 1 mg/mL, purified immunoglobulin

• Synonym(s): Anti-MDC1, P2B11, Anti-Nuclear factor with BRCT domains1, Anti-mediator of DNA damage checkpoint 1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: P2B11, monoclonal
• form: buffered aqueous glycerol solution
• mol wt: antigen predicted mol wt 184 kDa
• species reactivity: human, mouse, bovine, chimpanzee
• concentration: 1 mg/mL
• technique(s): indirect immunofluorescence: suitable; western blot: suitable
• isotype: IgG1
• NCBI accession no.: NP_001010833.2
• UniProt accession no.: Q14676
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: mouse ... MDC1(240087)

Description

General description

Mediator of DNA damage checkpoint 1 (MDC1) is a nuclear protein that in humans is encoded by the MDC1 gene mapped to chromosome 6p21.33. The encoded protein is widely expressed in various types of tissues and organs. MDC1 is characterized with two BRCA1 C terminus (BRCT) domain, an N-terminal phosphoamino-acid-binding motif called a forkhead-associated (FHA) domain and large central proline/serine/threonine-rich repeat (PST) domain.

Specificity

Detects ~184 kDa. This antibody recognizes MDC1 at and around the N-terminus.

Immunogen

GST-tagged recombinant protein corresponding to mouse MDC1 ar and around the N-termius

Biochem/physiol Actions

Mediator of DNA damage checkpoint 1 (MDC1) plays a vital role in stimulation of the intra–S phase and G2-M phase checkpoints of the cell cycle in response to DNA damage. Polymorphism of the gene has been associated with the development of both the serum LDL-cholesterol concentration and hyper–LDL-cholesterolemia. Mammalian MDC1/ nuclear factor with BRCT domain 1 (NFBD1) interacts with phospho-H2AX (γH2AX) and plays a key role in DNA damage response by facilitating normal radioresistance and efficient accumulation of DNA-damage-response proteins on damaged chromatin.

Features and Benefits

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Physical form

Solution in PBS, pH 7.4, 50% glycerol, and 0.09% sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable