SAB5100001

Imprint^® ChIP-Validated Monoclonal Anti-p53 (DO-1) antibody produced in mouse

clone DO-1, purified immunoglobulin

• Synonym(s): Imprint^® Monocolonal Anti-p53 antibody produced in mouse, Anti-LFS1, Anti-P53, Anti-TP53, Anti-TRP53, Anti-tumor protein p53
• UNSPSC Code: 12352200
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: DO-1, monoclonal
• form: buffered aqueous solution
• species reactivity: human
• technique(s): ChIP: suitable; western blot: suitable
• isotype: IgG2a
• UniProt accession no.: P04637
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TP53(7157)

Description

General description

The optimal amount of antibody per ChIP assay should be empirically determined by the end-user depending upon the amount of chromatin used per reaction. We recommend testing 1-5 μg per ChIP assay.

The tumor suppressor protein p53 responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Wildtype p53 protein is expressed at a low level in normal cells but often mutated and expressed at a high level in a variety of transformed cell lines, where it is believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity.

Immunogen

recombinant human wild type p53 ^1-5.

Application

Suitable for Chip and western blot.

Quality

ChIP Validation

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20°C in working aliquots. Repeated freezing and thawing or storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Other Notes

View ChIP Validation Data for lot number: 032M4761

Legal Information

Imprint is a registered trademark of Sigma-Aldrich Co. LLC

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable