SAB4700495

Monoclonal Anti-CD62P, low endotoxin antibody produced in mouse

clone AK4, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-P-selectin, Anti-SELP
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: AK4, monoclonal
• form: buffered aqueous solution
• species reactivity: nonhuman primates, human
• concentration: 1 mg/mL
• technique(s): flow cytometry: suitable
• impurities: ≤1 EU/μg endotoxin (LAL test)
• isotype: IgG1
• NCBI accession no.: NM_003005.3
• UniProt accession no.: P16109
• shipped in: wet ice
• storage temp.: 2-8°C
• Gene Information: human ... SELP(6403)

Description

General description

CD62P (P-selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.

Specificity

The antibody AK4 recognizes CD62P (P-selectin), a 140 kD single chain type I transmembrane glycoprotein present in secretory alpha-granules in platelets, in Weibel-Palade bodies in endothelial cells and in megakaryocytes; it is relocated to the plasma membrane upon activation.

Immunogen

Human platelets

Application

The reagent is designed for Flow Cytometry analysis. Suggested working dilution for Flow Cytometry is 1.5 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Solution in azide free phosphate buffered saline, pH 7.4; 0.2 um filter sterilized. Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable