SAB4700181

Monoclonal Anti-CD44-Biotin antibody produced in mouse

clone MEM-85, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-Pgp-1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: biotin conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: MEM-85, monoclonal
• form: buffered aqueous solution
• species reactivity: human
• concentration: 1 mg/mL
• technique(s): flow cytometry: suitable
• isotype: IgG2b
• NCBI accession no.: NM_000610.3
• UniProt accession no.: P16070
• shipped in: wet ice
• storage temp.: 2-8°C
• Gene Information: human ... CD44(960)

Description

General description

The CD44 (cell-surface glycoprotein) gene with 20 exons is mapped to human chromosome 11p13. The gene codes for a transmembrane glycoprotein.

The antibody MEM-85 reacts with both cell surface-expressed and soluble form of CD44 antigen (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes.

Immunogen

Leukocytes of a patient suffering from LGL Type Leukaemia.

Application

The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 1:1000. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Biochem/physiol Actions

CD44 (cell-surface glycoprotein) is involved in cell-cell and cell-extracellular matrix interactions. It plays an important role in lymphocyte homing and lymphocyte activation. It also acts as a metastasis suppressor gene for prostatic cancer. Overexpression of CD44 leads to esophageal squamous cell carcinoma (ESCC). CD44 inhibits macrophage multinucleation by interacting with its ligands, hyaluronic acid, chondroitin sulfates and osteopontin. 100kDa form of CD44, facilitates the binding of poliovirus to HeLa cells. In addition, it also implicated in the infection of mononuclear phagocytes by human immunodeficiency virus (HIV).

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Solution in phosphate buffered saline, pH 7.4, with 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable