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Key Documents

SAB4504713

Sigma-Aldrich

Anti-phospho-MKP-1/2 (pSer296/318) antibody produced in rabbit

affinity isolated antibody

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

39 kDa

species reactivity

mouse, human, rat

concentration

~1 mg/mL

technique(s)

ELISA: 1:5000
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000

NCBI accession no.

P28562
Q13115

UniProt accession no.

P28562

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer296/pSer318)

Gene Information

human ... DUSP1(1843) , DUSP4(1846)

General description

MKP1 (mitogen-activated protein kinase phosphatase 1)/DUSP1 (dual specificity phosphatase 1) is a dual specificity phosphatase. DUSP1 is also called as CL100, 3CH134 and Erp. MKP1 is located in the nucleus and is expressed in several tissues. The MKP gene is located on human chromosome 5q35.

Immunogen

The antiserum was produced against synthesized peptide derived from human MKP-1/2 around the phosphorylation site of Ser296/318.

Immunogen Range: 261-310

Biochem/physiol Actions

In mast cells, MKP1 (mitogen-activated protein kinase phosphatase 1) blocks the functions of extracellular signal-regulated kinase (Erk)‐1/2 by glucocorticoid. It controls axon branching promoted by BDNF (brain-derived neurotrophic factor). These results suggest that that DUSP4 is a critical regulator of the growth and invasion of triple-negative breast cancer cells. DUSP4 specifically regulates extracellular regulated kinase (ERK). DUSP4 is an important tumor suppressor gene in these aggressive breast cancers.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
118372

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Conditional expression of the mitogen-activated protein kinase (MAPK) phosphatase MKP-1 preferentially inhibits p38 MAPK and stress-activated protein kinase in U937 cells
Franklin CC and Kraft AS
The Journal of Biological Chemistry, 272(27), 16917-16923 (1997)
MAP kinase phosphatases
Theodosiou A and Ashworth A
Genome Biology (2002)
Analysis of phosphatases in ER-negative breast cancers identifies DUSP4 as a critical regulator of growth and invasion
Mazumdar A, et al.
Breast Cancer Research and Treatment, 158(3), 441-454 (2016)
Glucocorticoids inhibit MAP kinase via increased expression and decreased degradation of MKP-1
Kassel O, et al.
The Embo Journal, 20(24), 7108-7116 (2001)
The MAP kinase phosphatase MKP-1 regulates BDNF-induced axon branching
Jeanneteau F, et al.
Nature Neuroscience, 13(11), 1373-1379 (2010)
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