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Key Documents

SAB4502333

Sigma-Aldrich

Anti-PRKAB1 antibody produced in rabbit

affinity isolated antibody

Synonym(s):

5′-AMP-activated protein kinase subunit β-1, AMPK, AMPK subunit β-1, AMPKb, PRKAB1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 30 kDa

species reactivity

mouse, human, rat

concentration

~1 mg/mL

technique(s)

ELISA: 1:10000
western blot: 1:500-1:1000

NCBI accession no.

Q9Y478

UniProt accession no.

Q9Y478

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... PRKAB1(5564)

General description

Anti-PRKAB1 Antibody detects endogenous levels of total PRKAB1 protein.

Immunogen

The antiserum was produced against synthesized peptide derived from human PRKAB1.

Immunogen Range: 10-59

Application

Anti-PRKAB1, N-Terminal antibody produced in rabbit is suitable for indirect ELISA and western blot applications.

Biochem/physiol Actions

PRKAB1 (protein kinase, AMP-activated, β 1 non-catalytic subunit) is a non-catalytic, β subunit of AMP-activated protein kinase (AMPK). It acts as a metabolic sensor for AMP levels. It plays a key role in energy sensor during the response to energy demand and supply. It regulates cell metabolism. It increases the glycolytic flux by H202 which increase intracellular NADPH content. The activity of PRKAB1 is controlled by decreasing concentrations of adenosine triphosphate (ATP) and increasing AMP concentrations.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
3112821

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Jonathan S Oakhill et al.
Science (New York, N.Y.), 332(6036), 1433-1435 (2011-06-18)
The adenosine monophosphate (AMP)-activated protein kinase (AMPK) regulates whole-body and cellular energy balance in response to energy demand and supply. AMPK is an αβγ heterotrimer activated by decreasing concentrations of adenosine triphosphate (ATP) and increasing AMP concentrations. AMPK activation depends
Cuilan Li et al.
Molecular cancer, 13, 49-49 (2014-03-08)
AMP-activated protein kinase (AMPK) is a key energy sensor that is involved in regulating cell metabolism. Our previous study revealed that the subunits of the heterotimeric AMPK enzyme are diversely expressed during ovarian cancer progression. However, the impact of the
Shi-Bei Wu et al.
Biochimica et biophysica acta, 1822(2), 233-247 (2011-10-18)
We report that the energy metabolism shifts to anaerobic glycolysis as an adaptive response to oxidative stress in the primary cultures of skin fibroblasts from patients with MERRF syndrome. In order to unravel the molecular mechanism involved in the alteration
D Stapleton et al.
FEBS letters, 409(3), 452-456 (1997-06-16)
The AMP-activated protein kinase (AMPK) consists of catalytic alpha and non-catalytic, beta and gamma (38 kDa) subunits and is responsible for acting as a metabolic sensor for AMP levels. There are multiple genes for each subunit and we find that

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