SAB4300695

Anti-CDK4 antibody produced in rabbit

affinity isolated antibody

• Synonym(s): Anti-CMM3, Anti-PSK-J3
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: ~34 kDa
• species reactivity: human
• concentration: 1 mg/mL
• technique(s): western blot: 1:500-1:1000
• isotype: IgG
• immunogen sequence: (L-H-K-D-E)
• NCBI accession no.: NM_000075.2
• UniProt accession no.: P11802
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CDK4(1019)

Description

General description

The antibody detects endogenous levels of total CDK4 protein.

Immunogen

Peptide sequence around aa. 295-299 (L-H-K-D-E), according to the protein NP_000066.1

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Features and Benefits

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Target description

Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins . The inactive ternary complex of CDK4/cyclin D and p27 Kip1/Cip1 requires extracellular mitogenic stimuli for the release and degradation of p27, which affects progression through the restriction point and pRb-dependent entry into S-phase . The active complex of CDK4/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression . In HeLa cells, upon UV irradiation, upregulation of p16 INK4A association with CDK4/cyclin D3 leads to a G2 delay, implicating CDK4/cyclin D3 activity in progression through the G2-phase of the cell cycle .

Physical form

Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable