Francisella tularensis is a Gram-negative bacteria that causes tularemia. Tularemia is a highly contagious and fatal zoonotic disease isolated from hundreds of animal species with severe clinical symptoms, including skin and gastrointestinal lesions and pneumonia.1-3 F. tularensis is listed as a category A biothreat agent by the CDC due to its aerosol and water-borne dissemination, high infectivity and it potential to high morbidity and mortality in humans. In nature F. tularensis can persist for long periods at low temperatures in animal carcasses, soil or water. There are three major F. tularensis group classified according to their geographic and virulence status.2-3
Specificity
Monoclonal Anti-F. tularensis LPS specifically recognizes Francisella tularensis and does not cross react with Escherichia coli bacteria.
Application
The antibody may be used in various immunochemical techniques including Immunoblot, Immunofluorescence, and ELISA. Detection of the F. tularensis LVS (live vaccine strain) in Immunoblotting is specifically inhibited by the immunogen. The recombinant antibody is composed of Human Fc chain and variable regions from rabbit origin, therefor it is recommended to use Anti-Human Fc antibody as the secondary antibody for detection.
Biochem/physiol Actions
F. tularensis virulence is unique and researchers are trying to understand the bacteria pathogenesis, mechanism of action and discover its virulence factors since it does not appear to secrete any toxins.2-4 Furthermore, its′ LPS has an unusual structure and is ~1000-fold less potent than most Gram-negative bacteria LPS and does not activate cells via Toll-like receptor 4 (TLR4). 3-4 Hence F. tularensis LPS can be use as good target for generation of specific antibodies and potential vaccine for F. tularensis infections.1,5
Physical form
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
Storage and Stability
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Francisella tularensis (Ft), the causative agent of lethal tularemia, is classified as a category A biological warfare threat agent. While Ft infection is treatable by antibiotics, many failed antibiotic treatments were reported, highlighting the need for effective new treatments. It
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