SAB4200802

Anti-Epidermal Growth Factor (EGF) Receptor antibody, Mouse monoclonal

clone F4, purified from hybridoma cell culture

• Synonym(s): Anti-EGFR, Anti-Proto-oncogene c-ErbB-1, Anti-Receptor tyrosine-protein kinase erbB-1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: F4, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~170 kDa
• species reactivity: human
• packaging: antibody small pack of 25 μL
• concentration: ~1.0 mg/mL
• technique(s): immunoblotting: 0.3-0.6 μg/mL using human epidermoid carcinoma A431 cell line extract; immunofluorescence: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• isotype: IgG1
• UniProt accession no.: P00533
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... EGFR(1956)

Description

General description

Epidermal growth factor receptor (EGFR), also known as Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1 or HER-1, is an integral cell membrane protein which spans the membranes of a wide range of normal and malignant epithelial cells. EGFR is a tyrosine-specific protein kinase domain, it has an extracellular region which binds EGF and an intra-cellular carboxy-terminal region which possesses the tyrosine kinase activity.

Monoclonal Anti-Epidermal Growth Factor (EGF) Receptor (mouse IgG1 isotype) is derived from the F4 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse.

Immunogen

peptide corresponding to the C-terminal region of human EGF receptor (EGFR) (GeneID 1956), coupled to KLH

Application

Anti-Epidermal Growth Factor (EGF) Receptor antibody, Mouse monoclonal has been used in:

• immunoblotting
• immunoprecipitation
• immunohistochemistry
• immunofluorescence

Monoclonal Anti-Epidermal Growth Factor (EGF) Receptor recognizes intracellular domain of the EGF receptor. The antibody is recommended to use in various immunological techniques, including Immunobloting (∼170 kDa), Immunoprecipitation, Immunohistochemistry and Immunofluorescence.

Biochem/physiol Actions

Epidermal growth factor (EGF) binding to its specific EGFR, results in an increased DNA synthesis initiating a signaling cascades including cell proliferation, differentiation and repair of damaged epithelial tissue. The EGFR has a half-life of approximately ~10 hours in human fibroblasts, but in the presence of EGF this value is reduced to about 1hour. A close similarity has been found between the sequence of the v-erb-B oncogene and the cytoplasmic transmembrane domain of the EGFR (truncated EGFR). Overexpression or mutation of EGFR may enhance DNA repair mechanisms, thereby contributing to radio- and chemotherapy resistance. High levels of EGFR have been identified in sarcomas, gliomas, gynecological, breast, bladder and lung tumors.

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable