SAB4200789

Anti-Human Lambda Light Chains (Bound and Free)-Peroxidase antibody, Mouse monoclonal

clone HP-6054, purified from hybridoma cell culture

• Synonym(s): Anti-Human immunoglobulin lambda light chain
• UNSPSC Code: 12352200
• NACRES: NA.46

Properties

• biological source: mouse
• conjugate: peroxidase conjugate
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: HP-6054, monoclonal
• form: lyophilized powder
• species reactivity: human
• concentration: ~2 mg/mL
• technique(s): direct ELISA: 1:16,000-1:32,000 using using 2 μg/ml human IgG lambda light chains for coating.
• isotype: IgG2a
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Anti-Human Lambda Light Chains (Bound and Free)- Peroxidase antibody, Mouse monoclonal (mouse IgG2a isotype) is derived from the HP-6054 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified human IgG myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads. Immunoglobulins are composed of two heavy and two light polypeptide chains held together by noncovalent forces and usually by interchain disulfide bridges. The various types of human (and other mammalian) immunoglobulins contain one of the two existing light chain types, either κ or λ in which multiple structural differences are reflected in antigenic variety, mainly the N-terminal (variable) domain of the chains.

Immunogen

Purified human IgG myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads.

Application

Direct ELISA: a working dilution of 1:16,000-1:32,000 is recommended using 2 μg/mL human IgG lambda light chains for coating.

Biochem/physiol Actions

In monoclonal disorders, such as myeloma and macroglobulinemia, an increase in the level of a single immunoglobulin class can be accompanied by disproportionate increase in either λ or κ light chains. In many cases of B cell malignancy there is an increased production of light chains which are not accompanied with heavy chains. These circulate in the various body fluids (blood, cerebral spinal fluid, and tissues) and are also found in enormous amounts as free molecules in the urine (Bence Jones paraproteins).

Physical form

Supplied as a lyophilized powder.

Other Notes

In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

Disclaimer

For U.S. Customers: Contains mercury; Do not place in trash - dispose according to local, state, or federal laws.

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable