SAB4200771

Anti-Surfactant Protein D antibody, Mouse monoclonal

clone 10F6E12, purified from hybridoma cell culture

• Synonym(s): Anti-Collectin-7, Anti-Lung surfactant protein D, Anti-PSP-D, Anti-Pulmonary surfactant-associated protein D, Anti-SP-D
• UNSPSC Code: 12352200

Properties

• biological source: mouse
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 10F6E12, monoclonal
• form: buffered aqueous solution
• mol wt: ~43 kDa
• species reactivity: mouse, human
• concentration: ~1 mg/mL
• technique(s): immunoblotting: 2-4 μg/mL using mouse lung extract
• isotype: IgG1
• UniProt accession no.: P35247
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SFTPD(6441) ; mouse ... Sftpd(20390)

Description

General description

Surfactant Protein D (SFTPD) belongs to the collectin family of multimeric glycoproteins and exists as oligomeric structure or a trimeric subunit. The human SFTPD corresponds to a molecular weight of 150kDa and the trimer is close to 620 kDa. Anti-Surfactant Protein D antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the 10F6E12 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from mouse immunized with mouse recombinant SP-D.

Surfactant Protein D (SP-D) contains an N-terminal cysteine-rich domain, a collagenous domain, a neck region and a C-terminal carbohydrate recognition domain (CRD). The gene is located on human chromosome 14. SP-D is expressed in lungs and kidney.

Specificity

Anti-Surfactant Protein D antibody, Mouse monoclonal recognizes SP-D from human and mouse origin.

Immunogen

Mouse recombinant SP-D

Application

Anti-Surfactant Protein D antibody, Mouse monoclonal may be used in immunoblotting.

Biochem/physiol Actions

Surfactant Protein D (SFTPD) imparts pulmonary defense during fungal infection. It favors pulmonary inflammation. It recognizes the surface lipopolysaccharide (LPS) of pathogens and acts as first-line host defense molecule. It also recognizes the viral glycoprotein hemagglutinin (HA) present in viral surface. Polymorphism in the SFTPD gene may lead to decreased levels of the protein and high risk for chronic obstructive pulmonary disease (COPD).

Surfactant Protein D (SP-D) plays a critical role in the innate host defense and the modulation of inflammatory responses in infectious diseases. It aids the uptake and clearance of pathogens and apoptotic cells. SP-D maintains surfactant homeostasis. It reduces the surface tension at the air-liquid interface and blocks the lung from collapsing.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable