SAB4200765

Anti-tau antibody, Mouse monoclonal

clone tu53, purified from hybridoma cell culture

• Synonym(s): Anti-Microtubule-associated protein tau (MART), Anti-Neurofibrillary tangle protein, Anti-Paired helical filament-tau (PHF-tau), Anti-Tau-F
• UNSPSC Code: 12352202
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: tu53, monoclonal
• form: buffered aqueous solution
• mol wt: 46-60 kDa
• species reactivity: human, mouse
• concentration: ~1 mg/mL
• technique(s): immunoblotting: 5-10 μg/mL using mouse brain tissue extract; immunoprecipitation (IP): suitable
• isotype: IgG1
• UniProt accession no.: P10636
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MAPT(4137)

Description

General description

Anti-tau antibody, mouse monoclonal (mouse IgG1 isotype) is derived from the tu53 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB /C mice. Tau, also known as microtubule-associated protein tau (MAPT), belongs to microtubule-associated proteins (MAP) family. It consists of six isoforms in the neurons of the adult human brain. MAPT gene is located on the human chromosome at 17q21.31.

Specificity

Anti-tau antibody, Mouse monoclonal specifically recognizes human and mouse tau protein. The antibody recognizes all six isoforms of human tau.

Immunogen

Recombinant human tau protein expressed in HEK-293 cells

Application

Anti-tau antibody, mouse monoclonal may be used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Tau loses its microtubule-binding activity and aggregates into paired helical filaments (PHFs) during neurodegenerative disorders. It can undergo modifications such as hyperphosphorylation, nonenzymatic glycosylation and acetylation, which result in the generation of aberrant aggregates such as found in neurofibrillary tangles (NFTs) in Alzheimer′s disease. It is present abundantly in intraneuronal and glial fibrillar lesions formed during neurodegenerative disorders such as tauopathies and Alzheimer′s disease.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable