SAB4200752

Anti-Nuclease P1 antibody produced in rabbit

Ig fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-Deoxyribonuclease P1, Anti-Endonuclease P1
• UNSPSC Code: 12352202
• NACRES: NA.41

Properties

• biological source: rabbit
• antibody form: Ig fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: ~30 kDa
• concentration: ~1 mg/mL
• technique(s): immunoblotting: 1:10,000-1:20,000 using Nuclease P1 from Penicillium citrinum
• UniProt accession no.: P24289
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Nuclease P1 (Nuclease 5′-Nucleotidehydrolase, 3′- Phosphohydrolase, EC 3.1.30.1), also known as deoxyribonuclease P1 or endonuclease P1, is a zinc dependent endonuclease. It is isolated from Penicillium citrinum.

Immunogen

Nuclease P1 from Penicillium citrinum

Application

Anti-Nuclease P1 antibody produced in rabbit has been used in immunoblotting.

Biochem/physiol Actions

Nuclease P1 catalyzes the hydrolysis of 3′-5′ phosphodiester bonds in RNA and single-stranded DNA and 3′-phosphomonoester bonds in mono- and oligonucleotides terminated by a 3′-phosphate group without apparent bases specificity. The enzyme is used as a component of nuclease cocktail to generate single-stranded oligodeoxynucleotide (ODNs) samples for mass spectroscopy research for characterization of oligodeoxyribonucleotides. Nuclease P1 has also been widely used in the food industry to enhance or create flavor.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable