SAB4200737

Anti-MART-1 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-MART-1, Anti-MART1, Anti-MLANA, Anti-Melanoma antigen recognized by T-cells 1
• UNSPSC Code: 12352202
• NACRES: NA.41

Properties

• biological source: rabbit
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: ~13 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): immunoblotting: 2-4 μg/mL using human melanoma SK-Mel-28 cell line extract; immunofluorescence: 0.25-0.5 μg/mL using human melanoma SK-Mel-28 cell line; immunohistochemistry: 5-10 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human melanoma sections
• UniProt accession no.: Q16655
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MART-1(2315)

Description

General description

Anti-MART-1 is produced in rabbit using a synthetic peptide corresponding to a sequence from the internal region of human MART-1 protein, conjugated to keyhole limpet hemocyanin (KLH). Melanoma antigen recognized by T-cells 1 (MART-1), also known as melan-A antigen, LB39-AA or antigen SK29-AA, is a type III signal-anchor protein that is localized mainly to the endoplasmic reticulum, the trans-Golgi network and melanosomes. MART-1 protein is expressed specifically in normal skin, retina, melanocyte cell lines and melanomas. It is a melanoma antigen recognized by T-cells and belongs to the melanocyte differentiation markers.

Immunogen

synthetic peptide corresponding to a sequence from the internal region of human MART-1 protein, conjugated to KLH

Application

Anti-MART-1 antibody produced in rabbit has been used in:

• immunoblotting
• immunofluorescence
• immunohistochemistry

Biochem/physiol Actions

Melanoma antigen recognized by T-cells 1 (MART-1) is specifically recognized by human leukocyte antigen (HLA-A2) restricted tumor-infiltrating T lymphocytes (TIL-1) in melanoma patients and is suggested to be involved in tumor progression. It is also involved in pigmentation process and is suggested as a candidate marker for albinism.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable