SAB4200727

Anti-AKT2 antibody, Mouse monoclonal

clone AKT2(104), purified from hybridoma cell culture

• Synonym(s): Anti-PKB beta, Anti-Protein kinase Akt-2, Anti-RAC-beta serine/threonine-protein kinase
• UNSPSC Code: 12352202

Properties

• biological source: mouse
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: AKT2(104), monoclonal
• form: buffered aqueous solution
• mol wt: ~56 kDa
• species reactivity: rat, mouse, human
• concentration: ~1.0 mg/mL
• technique(s): immunoblotting: 10-20 μg/mL using human T lymphocyte Jurkat cell extract
• isotype: IgG2a
• UniProt accession no.: P31751
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... AKT2(208)

Description

General description

Anti-AKT2 antibody, Mouse monoclonal, (mouse IgG2a isotype) is derived from the AKT2(104) hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse. v-akt murine thymoma viral oncogene homolog 2 (AKT2) protein belongs to the subfamily of three closely related protein serine/threonine kinases. AKT2 is localized in the insulin-responsive tissues like adipose tissue, liver and skeletal muscle. It comprises of N-terminal regulatory pleckstrin homology (PH) domain, a central kinase domain with serine/threonine specificity and a C-terminal hydrophobic domain. It is mapped on the human chromosome at 19q13.2.

Specificity

Monoclonal Anti-AKT2 antibody specifically recognizes human, mouse and rat AKT2 and does not recognize AKT1 nor AKT3.

Immunogen

synthetic peptide from the internal region of human AKT2 protein, conjugated to KLH

Application

Anti-AKT2 antibody, Mouse monoclonal may be used in immunoblotting.

Biochem/physiol Actions

v-akt murine thymoma viral oncogene homolog 2 (AKT2) is a key mediator of the insulin signal transduction process and is required for glucose metabolism. AKT2 plays an important role in the phosphoinositide 3-kinase (PI3K) signaling pathway and is over-expressed in a variety of malignant tumors. it is considered to be a useful molecular marker for predicting the prognosis of meningioma and breast cancer. Due to its important role in neuroblastoma tumorigenesis, it can be used as a target for developing novel therapeutics for the treatment of clinically aggressive neuroblastoma.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Storage and Stability

Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable