SAB4200675

Monoclonal Anti-Multimerin-2 antibody produced in mouse

clone MM-5, purified from hybridoma cell culture

• Synonym(s): EMILIN-3, EMILIN3, ENDOGLYX-1, MMRN2
• UNSPSC Code: 51111800
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: MM-5, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~104 kDa
• species reactivity: human
• concentration: ~1 mg/mL
• technique(s): immunoblotting: 5-10 μg/mL using whole extract of human HepG2 cells.; immunofluorescence: 2-5 μg/mL using human HeLa cells
• isotype: IgG1
• UniProt accession no.: Q9H8L6
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MMRN2(79812)

Description

General description

Monoclonal Anti-Multimerin-2 (mouse IgG1 isotype) is derived from the hybridoma MM-5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Multimerin-2 (MMRN2) belongs to the EMI domain endowed (EDEN) protein family. It is located in the blood vessels and is a glycoprotein. MMRN2 gene is mapped on the human chromosome at 10q23.2.

Multimerin-2 (MMRN2) is an extracellular matrix glycoprotein. The protein contains a signal sequence, an amino-terminal EMI (cysteine-rich) domain and a carboxyl-terminal C1q (complement 1)-like domain. MMRN2 is also referred to as endoglyx-1.

Specificity

Monoclonal Anti-Multimerin-2 recognizes human Multimerin-2.

Immunogen

a recombinant protein of human Multimerin-2

Application

Monoclonal Anti-Multimerin-2 antibody produced in mouse may be used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

Multimerin-2 (MMRN2) negatively regulates the angiogenesis regulators vascular endothelial growth factor receptor 1 & 2 (VEGFR 1 & 2). It plays a role in the maintenance of blood vessels homeostasis, regulation of endothelial cell (EC) function and hence tumor growth arrest.

Multimerin-2 (MMRN2) regulates endothelial cell migration and neo-angiogenesis through interaction with VEGF-A (vascular endothelial growth factor-A).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable