SAB4200659

Monoclonal Anti-Glucokinase (GCK) antibody produced in mouse

clone GCK-20, purified from hybridoma cell culture

• Synonym(s): FGQTL3, GK, GLK, HHF3, HK4, HKIV, HXKP, LGLK, MODY2, glucokinase (hexokinase 4)
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: GCK-20, monoclonal
• form: buffered aqueous solution
• mol wt: ~50 kDa
• species reactivity: human
• concentration: ~1 mg/mL
• technique(s): flow cytometry: 10-20 μg/test using A549 cells; immunoblotting: 1.25-2.5 μg/mL using whole extract of HepG2 cells.; immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• isotype: IgG1
• UniProt accession no.: P35557
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... GCK(2645)

Description

General description

The gene encoding glucokinase (GCK) is alternatively spliced to generate three different forms of the enzyme; one found in the pancreas and two found in the liver. The enzyme is expressed as a monomer and possesses one glucose-binding site. The gene encoding GCK is localized on human chromosome 7.[1] Recombinant human liver glucokinase (Accession# NP_277042)) has a C-terminal FLAG tag and has 474 amino acid residues.

Immunogen

synthetic peptide corresponding to a sequence at the N-terminal region of human Glucokinase

Biochem/physiol Actions

Glucose is phosphorylated to glucose-6-phosphate by glucokinases. The main function of glucokinase (GCK) is to regulate carbohydrate metabolism. The enzyme triggers the release of insulin in β-cells of pancreas. It can be useful for studies including enzyme kinetics, activator screening and kinase selectivity. Mutation in the gene encoding this protein is associated with a rare form of diabetes and the protein may also have a role in type 2 diabetes.[1]

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable