SAB4200648

Monoclonal Anti-SIRT1 antibody produced in mouse

clone SIRT1-1, purified from hybridoma cell culture

• UNSPSC Code: 51111800
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: SIRT1-1, monoclonal
• form: buffered aqueous solution
• species reactivity: human
• concentration: ~1 mg/mL
• technique(s): flow cytometry: 5-10 μg/test using HeLa cells.; immunoblotting: 1-2 μg/mL using whole extracts of human Jurkat cells.; immunoprecipitation (IP): 5-10 μg using extracts of human Jurkat cells
• isotype: IgG2b
• UniProt accession no.: Q96EB6
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... Sirt1(23411)

Description

General description

Monoclonal Anti-Sirt1 (mouse IgG2b isotype) is derived from the hybridoma SIRT1-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human Sirt1 recombinant protein. Sirtuin 1 is a member of mammalian Sir2 gene family. It is present in the nucleus.

Immunogen

human Sirt1 recombinant protein

Application

Monoclonal Anti-SIRT1 antibody produced in mouse has been used in various immunochemical techniques including immunoblotting, immunoprecipitation and flow-cytometry.

Biochem/physiol Actions

Sirtuin 1 (Sirt1) is an NAD-dependent deacetylase and regulates various transcription factors, such as p53, FOX (forkhead box), nuclear factorΚB, myocyte enhancer factor-2 (Mef2) and peroxisome proliferator-activated receptor γ coactivator 1 α (PGC1α). Sirt1 deacetylates and downregulates p53. Increased activity of Sirt1 in culture cells reduces p53 transcriptional activity and p53-mediated apoptosis in response to radiation or oxidative stress. In addition, it also deacetylates and represses the activity of the forkhead transcription factor FOXO3a, other mammalian forkhead factors and reduces forkhead dependent apoptosis. Sirt1 plays a key role in myocyte differentiation by binding to the myoD cofactor, pCAF. It has been shown that Sirt1 modifies chromatin and silences transcription of integrated reporter genes via histone deacetylation. Sirt1 provides protection against neurodegeneration in models for Alzheimer′s disease and amyotrophic lateral sclerosis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable