SAB4200640

Monoclonal Anti-CHD5 antibody produced in rat

clone 5A10, purified from hybridoma cell culture

• Synonym(s): Anti-ATP−dependent helicase CHD5, Anti-CHD−5, Anti-CHD5, Anti-RP1−233K16.2, Anti-chromodomain helicase DNA binding protein 5, Anti-chromodomain−helicase−DNA−binding protein 5
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rat
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 5A10, monoclonal
• form: buffered aqueous solution
• mol wt: ~250 kDa
• species reactivity: monkey, mouse, human
• concentration: ~1 mg/mL
• technique(s): flow cytometry: 2.5-5 μg/test using using HeLa cells.; immunoblotting: 1.0-2.0 μg/mL using using whole extracts of L929 cells.; immunofluorescence: 5-10 μg/mL using using HeLa cells.
• isotype: IgG2a
• UniProt accession no.: Q8TDI0
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: Monkey ... CHD5(720712) ; human ... CHD5(26038) ; mouse ... 269610(Chd5)

Description

General description

Monoclonal Anti-CHD5 (rat IgG2a isotype) is derived from the hybridoma 5A10 produced by the fusion of mouse myeloma cells and lymph node cells from rat immunized with a synthetic peptide corresponding to a sequence at the C-terminal region of human CHD5. Chromodomain helicase DNA(CHD) binding protein 5 (CHD5) is an ATP-dependent chromatin-remodeling factor. It is mainly expressed in neuronal tissues. CHD5 is a member of CHD family of proteins that is characterized with histone binding chromo (chromatin organization modifier) domains and sucrose non-fermentable 2 (SNF2)-related helicase/ATPase domains.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminal region of human CHD5 .

Application

Monoclonal Anti-CHD5 antibody produced in rat has been used in various immunochemical techniques including:

• immunoblotting
• flow cytometry
• immunocytochemistry
• immunohistochemistry

Biochem/physiol Actions

Chromodomain helicase DNA binding protein 5 (CHD5) regulates genes involved in neuronal function, cell-cycle control, and chromatin remodeling. Interestingly, CHD5 is frequently deleted in neuroblastoma and thus suggested as a tumor suppressor gene. Furthermore, non-conformist CHD5 promoter methylation was identified in several primary tumors.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable