SAB4200635

Monoclonal Anti-C19orf10 antibody produced in mouse

clone 15.3.3, purified from hybridoma cell culture

• Synonym(s): IL25, IL27, IL27w, Stromal cell-derived growth factor SF20, UPF0556 protein C19orf10, myeloid-derived growth factor (MYDGF)
• UNSPSC Code: 51111800
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 15.3.3, monoclonal
• form: buffered aqueous solution
• mol wt: ~17 kDa
• species reactivity: bovine, canine, human
• concentration: ~1 mg/mL
• technique(s): flow cytometry: suitable; immunoblotting: suitable; immunofluorescence: suitable
• isotype: IgG2a
• UniProt accession no.: Q969H8
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MYDGF(56005)

Description

General description

Monoclonal Anti-C19orf10 (mouse IgG2a isotype) is derived from the hybridoma 15.3.3 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the internal region of human C19orf10, conjugated to KLH. C19orf10 (chromosome 19 open reading frame 10) also known as IL25 (Interleukin - 25) and Stromal cell-derived growth factor SF20, is a secreted protein. This protein is an FLS (fibroblast-like synoviocytes)-derived protein and is discharged into the synovial fluid.

Immunogen

synthetic peptide corresponding to a sequence at the internal region of human C19orf10.

Application

Monoclonal Anti-C19orf10 antibody has been used in

• immunoblotting
• immunocytochemistry
• immunofluorescence
• flow cytometry

Biochem/physiol Actions

C19orf10 (chromosome 19 open reading frame 10) was overexpressed in approximately two-thirds of hepatocellular carcinoma (HCC) tissues compared to the adjacent non-cancerous liver tissues. It also regulates HCC cell line proliferation by activating the Akt/mitogen-activated protein kinase pathways.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable