SAB4200633

Monoclonal Anti-QPCT antibody produced in mouse

clone QP-2, purified from hybridoma cell culture

• Synonym(s): Glutaminyl-tRNA cyclotransferase (GCT), Glutamyl cyclase, QC, glutaminyl-peptide cyclotransferase, sQC
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: QP-2, monoclonal
• mol wt: ~40 kDa
• species reactivity: human
• concentration: ~1 mg/mL
• technique(s): flow cytometry: 5-10 μg/test using using HeLa cells.; immunoblotting: 2-4 μg/mL using using whole extracts of SK-Mel-28 cells.; immunofluorescence: 4-8 μg/mL using using SK-Mel-28 cells.
• isotype: IgG2a
• UniProt accession no.: Q16769
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... QPCT(25797)

Description

General description

Monoclonal Anti-QPCT (mouse IgG2a isotype) is derived from the hybridoma QP-2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Glutaminyl-peptide cyclotransferase (QPCT) is an enzyme present in the mammalian brain, porcine pituitary and human B lymphocytes. It is located on the human chromosome at 2p22.2.

Specificity

Monoclonal Anti-QPCT recognizes human QPCT.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminal region of human QPCT.

Application

Monoclonal Anti-QPCT antibody produced in mouse may be used in:

• immunoblotting
• flow cytometry
• immunofluorescence

Biochem/physiol Actions

Glutaminyl-peptide cyclotransferase (QPCT) catalyzes the post-translation modification of both glutaminyl precursors into N-terminal pyroglutamate (pGlu) and N-terminal glutamate cyclization. It is found to be involved in Alzheimer′s Disease (AD) pathogenesis and cognitive decline by QPCT-catalyzed pGlu- amyloid-β (Aβ) formation. Inhibition of QPCT prevents thyroid cancer metastasis. QPCT is also involved in other amyloidotic disorders such as familial British dementia (FBD) or familial Danish dementia (FDD).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For extended storage, freeze at -20 °Cin working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable