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SAB4200630

Sigma-Aldrich

Monoclonal Anti-SFRP1 antibody produced in mouse

clone FRP148, purified from hybridoma cell culture

Synonym(s):

Anti-FRP, Anti-FRP-1, Anti-FrzA, Anti-Secreted apoptosis-related protein 2 (SARP2), Anti-secreted frizzled-related protein 1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

FRP148, monoclonal

form

buffered aqueous solution

mol wt

antigen ~35 kDa

species reactivity

human

concentration

~1 mg/mL

technique(s)

flow cytometry: 10-20 μg/test using using WI-38 cells.
immunoblotting: 0.025-0.05 μg/mL using using whole extracts of WI-38 cells.

isotype

IgG2a

UniProt accession no.

Q8N474

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SFRP1(6422)

General description

The gene SFRP1 (secreted frizzled related protein 1) is mapped to human chromosome 8p22–p12, a region frequently deleted in breast cancer. It is highly expressed in normal breast tissue.

Immunogen

synthetic peptide corresponding to a sequence at the N-terminal region of human SFRP1.

Biochem/physiol Actions

Secreted Frizzled-related protein 1 (SFRP1) is a secreted inhibitor of Wnt signaling pathway. DNA damage or oxidative stress causes cellular senescence, which leads to the overexpression of sfrp1. This protein induces senescence phenotypes via the inactivation of Wnt signaling and activation of the retinoblastoma (Rb) pathway. Mutations or aberrant methylation in this gene impair the induction of senescence in several cancers, including breast cancer, bladder cancer, and colorectal tumorigenesis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Germaine M Caldwell et al.
Cancer research, 64(3), 883-888 (2004-02-12)
Regions of the short arm of chromosome 8 are deleted frequently in a range of solid tumors, indicating that tumor suppressor genes reside at these loci. In this study, we have examined the properties of the Wnt signaling antagonist secreted
Xiaobin Wang et al.
Oncology letters, 4(2), 334-338 (2012-07-31)
The aims of this study were to determine the methylation and expression status of secreted Frizzled-related protein 1 (SFRP1) in bladder cancer, to explore the mechanisms involved and to study the role of SFRP1 in the pathogenesis of bladder cancer.
J Veeck et al.
Oncogene, 25(24), 3479-3488 (2006-02-02)
The canonical Wnt signalling pathway plays a key role during embryogenesis and defects in this pathway have been implicated in the pathogenesis of various types of tumours, including breast cancer. The gene for secreted frizzled-related protein 1 (SFRP1) encodes a
David J Elzi et al.
Molecular and cellular biology, 32(21), 4388-4399 (2012-08-29)
Cellular senescence has emerged as a critical tumor suppressive mechanism in recent years, but relatively little is known about how senescence occurs. Here, we report that secreted Frizzled-related protein 1 (SFRP1), a secreted antagonist of Wnt signaling, is oversecreted upon

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