SAB4200616

Monoclonal Anti-FTL antibody from mouse

clone FTL1.1, purified immunoglobulin

• Synonym(s): Ferritin L subunit, LFTD;, NBIA3, ferritin light polypeptide
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: FTL1.1, monoclonal
• species reactivity: human
• concentration: 1 mg/mL
• technique(s): flow cytometry: 2-5 μg/test using using Jurkat cells.; immunoblotting: 5-10 μg/mL using using HepG2 total cell extracts.; immunofluorescence: 5-10 μg/mL using using HepG2 cells.
• isotype: IgG1
• UniProt accession no.: P02792
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... FTL(2512)

Description

General description

Monoclonal Anti-FTL (mouse IgG1 isotype) is derived from the hybridoma FTL1.1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal sequence of human FTL. Ferritin, a highly conserved iron-binding protein, is composed of two subunits, namely a 21 kDa heavy (H) chain and a 19 kDa light (L) chain. Ferritin light chain (FTL) is encoded by the gene mapped to human chromosome 19q13.33. FTL is highly found in liver and spleen.

Specificity

Monoclonal Anti-FTL recognizes human FTL.

Immunogen

synthetic peptide corresponding to an internal sequence of human FTL.

Application

Monoclonal Anti-FTL antibody from mouse may be used in:

• immunoblotting
• flow cytometry
• immunocytochemistry

Biochem/physiol Actions

Ferritin plays a vital role in maintaining iron homeostasis. The light chain ferritin, specifically mediates storage of iron in cells. Increased levels of FTL might lead to development of coronary artery disease (CAD) by regulating lipid oxidation within the vessel wall through reactive oxygen species generation. Furthermore, Mutation in the FTL gene causes an autosomal dominant, adult onset degenerative disease such as hereditary ferritinopathy (HF) or neuroferritinopathy. Interestingly, FTL has also been suggested as a tumor-associated macrophages related biomarker which is being utilized in prognosis prediction of breast cancer.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: No data available
• flash_point_c: No data available