SAB4200615

Monoclonal Anti-WDR49 antibody produced in mouse

~1.0 mg/mL, clone W49.1, purified immunoglobulin

• Synonym(s): Anti-WD repeat-containing protein 49, Anti-WDR49
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: W49.1, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~85 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): flow cytometry: 5-10 μg/test using Jurkat cells; immunoblotting: 20 μg/mL using total cell extracts of HEK-293T cells overexpressing WDR49; immunocytochemistry: suitable; immunofluorescence: 10-20 μg/mL using HeLa cells
• isotype: IgG1
• UniProt accession no.: Q8IV35
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... WDR49(151790)

Description

General description

Monoclonal Anti-WDR49 (mouse IgG1 isotype) is derived from the hybridoma W49.1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. WD repeat-containing protein 49 (WDR49) is expressed as two isoforms. It is located on the human chromosome at 3q26.1.

Specificity

Monoclonal Anti-WDR49 recognizes human WDR49.

Immunogen

synthetic peptide corresponding to an internal sequence of human WDR49

Application

Monoclonal Anti-WDR49 antibody produced in mouse may be used in:

• immunoblotting
• flow cytometry
• immunocytochemistry
• immunofluorescence

Biochem/physiol Actions

WD repeat-containing protein 49 (WDR49) is deleted in liver cancer as well as fatal, disomy 3 uveal melanoma. It may play a role as a tumor suppressor gene.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing,orstorage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable