SAB4200605

Anti-EB3 (C-terminal) antibody produced in rabbit

IgG fraction of antiserum

• Synonym(s): Anti-EBF3, Anti-EBF3-S, Anti-MAPRE3, Anti-RP/EB family, Anti-RP3, Anti-member 3, Anti-microtubule-associated protein
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~32 kDa
• species reactivity: rat, human, mouse
• technique(s): immunoblotting: 1:2,500-1:5,000 using lysates of mouse brain (S1 fraction).
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... EBF3(253738)

Description

General description

End-binding protein-3 (EB3), also known as microtubule (MT)-associated protein RP/EB family member 3 (MAPRE3), belongs to a distinct family of “plus-end tracking proteins” (+TIPs). In humans, EB1 family proteins consist of EB1, EB2 and EB3. EB proteins are components of the MT cytoskeleton and are greatly conserved. EB proteins contain an N-terminal calponin homology (CH) domain and a unique EB-like C-terminal motif. EB3 is preferentially expressed in the central nervous system (CNS) and binds to a CNS-specific adenomatous polyposis coli (APC) homolog APCL.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminus of human EB3, conjugated to KLH. The corresponding sequence is identical in rat and mouse EB3.

Application

Anti-EB3 (C-terminal) antibody produced in rabbit has been used in:

• immunoblotting
• immunofluorescence
• immunohistochemistry

Biochem/physiol Actions

End-binding protein-3 (EB3) plays an important role in regulating dynamics and organization of microtubules (MTs). EB3 is specifically expressed during myogenic differentiation and controls MTs dynamics at the cell cortex. Knockout of EB3 prevents myotubes formation by inhibiting myoblasts elongation and fusion. In neurons, EB3 is found to interact with scaffold protein ankyrin-G and postsynaptic density protein 95 (PSD95) and is required for stabilizing neuronal polarity in mature neurons and in determining neuronal dendritic morphology. EB3 is also involved in MT polymerization and cell migration. EB3 may be involved in stabilizing MTs and anchoring them to centrosomes during spindle formation.

Physical form

solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable