SAB4200596

Anti-NSF (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-N-ethylmaleimide-sensitive factor-like protein, Anti-N-ethylmaleimide-sensitive fusion protein, Anti-NEM-sensitive fusion protein, Anti-vesicle-fusing ATPase, Anti-vesicular-fusion protein NSF, SKD2
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~75 kDa
• species reactivity: mouse, rat, human
• concentration: ~1.0 mg/mL
• technique(s): immunoblotting: 1-2 μg/mL using extracts of SH-SY5Y cells.; immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin-embedded rat brain.; immunoprecipitation (IP): 10 μg using lysates of mouse brain (S1 fraction).; indirect immunofluorescence: 1-2 μg/mL using Neuro-2A cells.
• UniProt accession no.: P46459
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... NSF(4905)

Description

General description

N-ethylmaleimide-sensitive fusion protein (NSF) is a homotrimer and is made up of three domains such as N-terminal domain and two homologous adenosine triphosphate (ATP)-binding domains. It is located on the human chromosome at 17q21.31.

Specificity

Anti-NSF(C-terminal) specifically recognizes human, rat and mouse NSF.

Immunogen

synthetic peptide corresponding to a sequence located in the C-terminus of human NSF, conjugated to KLH. The corresponding sequence is highly conserved (single amino acid substitution) in rat and mouse NSF.

Application

Anti-NSF (C-terminal) antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunocytochemistry
• immunohistochemistry
• immunofluorescence

Biochem/physiol Actions

N-ethylmaleimide-sensitive fusion protein (NSF) is an essential component of the protein machinery responsible for various membrane fusion events, including inter-cisternal Golgi protein transport and the exocytosis of vesicles. NSF takes part in NSF-dependent membrane fusion in which interaction of two types of cytosolic proteins, NSF and α-, β- and γ- soluble NSF attachment protein (SNAP) isoforms, with the subcellular compartment-specific SNAP receptors (SNAREs) of the vesicle and target membranes. NSF is required in long-term potentiation (LTP), underlying the formation of long-term memory, by regulating the exocytosis of glutamate α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) glutamate receptor 2 (GluR2) at postsynaptic densities (PSD).

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use,store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable