SAB4200586

Anti-POLR2A antibody, Mouse monoclonal

clone RPOL-1, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-POLR2A antibody produced in mouse, Monoclonal Anti220kDa, Monoclonal AntiPOLR2, Monoclonal AntiPOLRA, Monoclonal AntiRPB1, Monoclonal AntiRPBh1, Monoclonal AntiRPO2, Monoclonal AntiRPOL2, Monoclonal AntiRpIILS, Monoclonal AntihRPB220, Monoclonal AntihsRPB1, Monoclonal Antipolymerase (RNA) II (DNA directed) polypeptide A
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: RPOL-1, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~250 kDa
• species reactivity: human
• concentration: ~1 mg/mL
• technique(s): immunoblotting: 1-2 μg/mL using nuclear extracts of human HeLa cells.
• isotype: IgG1
• UniProt accession no.: P24928
• shipped in: dry ice
• Storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... POLR2A(5430) ; mouse ... Polr2a(20020) ; rat ... Polr2a(363633)

Description

General description

Monoclonal Anti-POLR2A (mouse IgG1 isotype) is derived from the hybridoma RPOL-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Polymerase (RNA) II (DNA directed) polypeptide A (POLR2A) is the largest subunit of RNA polymerase II. It is mapped on the human chromosome at 17p13.1.

Specificity

Monoclonal Anti-POLR2A recognizes human POLR2A.

Immunogen

Synthetic peptide corresponding to the N-terminal region of human POLR2A, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey, bovine, dog and pig.

Application

Monoclonal Anti-POLR2A antibody produced in mouse may be used in immunoblotting.

Biochem/physiol Actions

Polymerase (RNA) II (DNA directed) polypeptide A (POLR2A) plays a role in the synthesis of messenger RNA in eukaryotes. It contains a carboxy-terminal domain composed of heptapeptide repeats that are essential for polymerase activity. POLR2A in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20°C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable