SAB4200583

Anti-Epsin1 (internal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): affinity isolated antibody
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~90 kDa
• species reactivity: rat, mouse, human
• concentration: ~1.0 mg/mL
• technique(s): immunoblotting: 1-2 μg/mL using extracts of SH-SY5Y cells and of mouse brain (S1 fraction).; indirect immunofluorescence: 2-4 μg/mL using NRK cells.
• UniProt accession no.: Q9Y6I3
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... EPN1(29924) ; mouse ... Epn1(13854) ; rat ... Epn1(117277)

Description

General description

Epsin1 is an endocytic adaptor protein. It contains an epsin N-terminal homology (ENTH) region, a single clathrin-binding LVDLD motif and two or three tandem ubiquitin-interacting motifs (UIMs). It is localized on the human chromosome at 19q13.42.

Specificity

Anti-Epsin1 (internal) specifically recognizes human,rat and mouse Epsin1.

Immunogen

synthetic peptide corresponding to an internal sequence of human epsin1 isoform A, conjugated to KLH. The corresponding sequence is identical in human espin1 isoforms B and C, and highly conserved (82% identity) in rat and mouse epsin1.

Application

Anti-Epsin1 (internal) antibody produced in rabbit may be used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

Epsin1 plays a central role in the clathrin-mediated internalization and degradation of ubiquitinated proteins. It interacts with Eps15 subunit of the clathrin adaptor AP2, clathrin and other accessory proteins. Epsin1 plays a role in the recruitment of ubiquitinated epidermal growth factor receptor (EGFR) into clathrin-coated pits. Epsin1 is required in the mitotic membrane and spindle organization. Overexpression of epsin1 is associated with fibrosarcoma cell invasion.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable