immunoblotting: 1-2 μg/mL using Lysates of HEK-293T cells overexpressing human Msi-1. immunoprecipitation (IP): 5-10 μg using HEK-293T cells overexpressing human Msi-1. indirect immunofluorescence: 5-10 μg/mL using HeLa cells.
Musashi-1 (Msi-1) is highly expressed in neural stem/progenitor cells (NS/PCs). MSI1 gene is mapped to human chromosome 12q24.
Immunogen
synthetic peptide corresponding to an internal sequence of human Msi-1, conjugated to KLH. The corresponding sequence is identical in rat and highly conserved (94% identity) in mouse Msi-1.
Application
Anti-Msi-1 antibody produced in rabbit has been used in:
immunoblotting
immunoprecipitation
immunofluorescence
Biochem/physiol Actions
Musashi-1 (Msi-1) is a neural RNA-binding protein that regulate translation of target mRNAs. It is associated with maintenance and asymmetric cell division of neural stem cells. Msi-1 binds to the 3′-untranslated region (UTR) of its target mRNAs in neural stem/progenitor cells (NS/PCs), repressing their translation, and interfering with NS/PC differentiation thus maintaining NP/PCs in the undifferentiated state. Loss of Msi-1 function disrupts the balance between germ-line stem cell renewal and differentiation, causing premature differentiation. Msi-1 has been shown to regulate the Notch and Wnt signaling pathway. Upregulation of Msi-1 augments Notch signaling, via translational repression of the Notch signaling antagonist m-Numb, thereby inhibiting the differentiation of neural stem cells into neurons. Msi-1 is also expressed in the nucleus during the early neural differentiation of mouse embryonic stem cells (ESCs) and found to act synergistically with Lin28 as a novel cofactor for the blockade of let-7 family miRNA biogenesis. Expression of Msi1- has been detected in several human cancers including brain tumors, suggesting its role in oncogenic development.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
RNA-binding protein Musashi family: roles for CNS stem cells and a subpopulation of ependymal cells revealed by targeted disruption and antisense ablation
Sakakibara S, et al.
Proceedings of the National Academy of Sciences of the USA, 99(23), 15194-15199 (2002)
The neural RNA-binding protein Musashi1 translationally regulates mammalian numb gene expression by interacting with its mRNA
Imai T, et al.
Molecular and Cellular Biology, 21(12), 3888-3900 (2001)
The RNA-binding protein Musashi1 affects medulloblastoma growth via a network of cancer-related genes and is an indicator of poor prognosis
Vo DT, et al.
The American Journal of Pathology, 181(5), 1762-1772 (2012)
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