SAB4200573

Anti-Claudin-4 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-CLDN4, Anti-CPE-R, Anti-CPER, Anti-CPETR, Anti-CPETR1, Anti-WBSCR8, Anti-hCPE-R
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~19 kDa
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~1.0 mg/mL
• technique(s): immunoblotting: 1.5-3.0 μg/mL using Extracts of HEK-293T cells overexpressing human claudin-4.
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CLDN4(1364)

Description

General description

Claudin-4 is heterogeneously expressed in various tissues. It is enriched in endothelial cells (ECs), including the blood-brain barrier, and is also expressed in liver, gut and kidney. Claudin-4 is a major constituent of tight junctions.

Specificity

Anti-Claudin-4 specifically recognizes humanclaudin-4.

Immunogen

synthetic peptide corresponding to an internal sequence of human claudin-4, conjugated to KLH. The corresponding sequence is highly conserved in mouse claudin-4 (89% identity) and in rat claudin-4 (84% identity).

Application

Anti-Claudin-4 antibody produced in rabbit may be used in immunoblotting.

Biochem/physiol Actions

Claudin-4 expression is deregulated in several cancers, with aberrant expression in ovarian, breast, prostate, gastric and pancreatic carcinomas.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable