SAB4200569

Anti-COG4 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-CDG2J, Anti-COD1, Anti-COG complex subunit 4, Anti-COMPONENT OF OLIGOMERIC GOLGI COMPLEX 4
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~84 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): indirect immunofluorescence: 2.5-5.0 μg/mL using human HeLa cells.; western blot: 2.5-5.0 μg/mL using whole extracts of hamster CHO cells.
• UniProt accession no.: Q9H9E3
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... COG4(25839)

Description

General description

Conserved oligomeric Golgi complex 4 (COG4) is a member of the conserved oligomeric Golgi (COG) complex. COG complex is an evolutionarily conserved multi-subunit protein complex. COG4 gene is mapped on the human chromosome at 16q22.1.

Specificity

Anti-COG4 recognizes human and hamster COG4.

Immunogen

peptide corresponding to the C-terminal region of human COG4, conjugated to KLH. The corresponding sequence is identical in bovine, dog and monkey, and differs by one amino acid in rat and mouse COG4.

Application

Anti-COG4 (C-terminal) antibody produced in rabbit may be used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

Conserved oligomeric Golgi complex 4 (COG4) is needed for normal Golgi function. It is implicated in Golgi soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) pairing and Golgi-to-endoplasmic reticulum (ER) retrograde transport via its interaction with the Sec1/Munc18 (SM) protein Sly1. Defects in the COG4 gene may be a cause of congenital disorder of glycosylation type II (CDG2).

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable