SAB4200564

Anti-AXIN2 antibody, Mouse monoclonal

clone AX13, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-AXIN2 antibody produced in mouse, Monoclonal Anti-G0/G1 switch regulatory protein 24, Monoclonal Anti-G0S24, Monoclonal Anti-Growth factor-inducible nuclear protein NUP475, Monoclonal Anti-NUP475, Monoclonal Anti-RNF162A, Monoclonal Anti-TIS11, Monoclonal Anti-TIS11A, Monoclonal Anti-Zinc finger protein 36 homolog, Monoclonal Anti-tristetraprolin, Monoclonal Anti-zinc finger protein 36, C3H type, homolog (mouse), Monoclonal Anti-zinc finger protein, C3H type, 36 homolog (mouse)
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: AX13, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~100 kDa
• species reactivity: human
• technique(s): western blot: 0.5-1.0 μg/mL using total cell extracts of HEK-293T cells overexpressing AXIN2.
• isotype: IgG1
• UniProt accession no.: Q9Y2T1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... AXIN2(8313)

Description

General description

Monoclonal Anti-AXIN2 (mouse IgG1 isotype) is derived from the hybridoma AX13 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Axis inhibition protein 2 (AXIN2) is a negative regulator of Wnt signaling and a putative tumor suppressor gene. It is located on the human chromosome at 17q24.1.

Specificity

Monoclonal Anti-AXIN2 recognizes human AXIN2.

Immunogen

synthetic peptide corresponding to a sequence at the N-terminal region of human AXIN2. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

Application

Monoclonal Anti-AXIN2 antibody produced in mouse may be used in immunoblotting.

Biochem/physiol Actions

Axis inhibition protein 2 (AXIN2) functions as a scaffold protein that takes part in a multiprotein complex that facilitates β-catenin phosphorylation as well as its degradation. It itself is activated by Wnt/T-cell factor signaling, resulting in a negative-feedback loop, which regulates the duration and intensity of Wnt/T-cell factor activation. AXIN2 might be involved in human cancer pathogenesis. Mutations in the AXIN2 gene are associated with tooth agenesis, colorectal carcinomas, hepatocellular carcinomas, hepatoblastomas, melanomas, gastric cancer and ovarian endometrioid adenocarcinomas. Axin2 functions as an essential regulator of remyelination and thus has been indicated as a possible checkpoint in this process.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable