SAB4200556

Anti-BMP4 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-BMP2B, Anti-BMP2B1, Anti-Bone morphogenetic protein 4, ZYME, Anti-MCOPS6, Anti-OFC11
• UNSPSC Code: 51111800
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~55 kDa
• species reactivity: human
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~1.0 mg/mL
• technique(s): immunohistochemistry: 20 μg/mL using formalin-fixed, paraffin-embedded human colon.; immunoprecipitation (IP): 10 μg using lysates of CHO cells overexpressing human BMP4.; western blot: 1-2 μg/mL using extracts of CHO cells overexpressing human BMP4.
• UniProt accession no.: P12644
• application(s): research pathology
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... BMP4(652)

Description

General description

Bone morphogenetic proteins (BMPs) are members of the TGF-β superfamily. BMP4, also known as ZYME or BMP2B, is encoded by the gene mapped to human chromosome 14q22.2. BMPs are expressed in localized patterns during embryonic mouse brain development.

Specificity

Anti-BMP4 specifically recognizes human BMP4.

Immunogen

synthetic peptide corresponding to an internal sequence of human BMP4, conjugated to KLH. The corresponding sequence is highly conserved (single amino acid substitution) in rat and mouse BMP4.

Application

Anti-BMP4 antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunohistochemistry

Biochem/physiol Actions

Bone morphogenetic proteins (BMPs) were originally identified as growth factors that can induce bone and cartilage formation. Various members of the BMP family are involved in a large variety of functions during development and in cancer. BMP4 is implicated in regulation of cell proliferation, differentiation, apoptosis, adult tissue homeostasis and cell fate determination during development. Specifically, BMP4 has been shown to regulate mesodermal cell commitment to the hematopoietic cell lineage during early embryogenesis. BMP4 is crucial for development since knockout mice die early in embryogenesis. Overexpression of BMP4 has been reported in several cancers, including melanoma, hepatocellular carcinoma, adenocarcinoma and colorectal cancer and is frequently accompanied by an enhanced cell migratory and invasive potential of the tumors.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable