SAB4200554

Anti-LIN28 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-LIN28A, Anti-RNA-binding protein LIN-28, Anti-lin-28 homolog A (C. elegans), Anti-protein lin-28 homolog A, Anti-zinc finger CCHC domain-containing protein 1, Anti-zinc finger, CCHC domain containing 1, CSDD1, ZCCHC1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~25 kDa
• species reactivity: mouse, human
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 1-2 μg using lysates of human NT2/D1 cells.; western blot: 1-2 μg/mL using whole extracts of human NT2/D1 cells.; western blot: 4-8 μg/mL using whole extracts of mouse P19 cells.
• UniProt accession no.: Q9H9Z2
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... LIN28A(79727)

Description

General description

LIN28 is an evolutionarily conserved RNA-binding protein. It is located on the human chromosome at 1p36.11.

Specificity

Anti-LIN28 (N-terminal) recognizes human and mouse LIN28.

Immunogen

synthetic peptide corresponding to the N-terminal region of human LIN28A, conjugated to KLH. The corresponding sequence is identical in monkey and dog and differs by a single amino acid in rat and mouse.

Application

Anti-LIN28 (N-terminal) antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

LIN28 is involved during development, pluripotency and oncogenesis. It also plays a role in metabolism, proliferation, differentiation and tumorigenicity. LIN28 acts as a post-transcriptional regulator of the biogenesis of a group of miRNAs, such as let-7 family of miRNAs. Lin28 binds to the loop regions of miRNA precursors, leading to inhibition of their processing into mature miRNAs, and/or induction of uridylation of the precursors that are subsequently degraded. Lin28 was also found to act as a translational modulator, probably by recruiting RNA helicase A (RHA) as a co-factor to enhance the translation of Lin28 target mRNAs. Overexpression of LIN28 is associated with cell proliferation and metastasis in human neck and head cancer.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable