SAB4200546

Anti-SLAMF6 antibody, Mouse monoclonal

clone SF24, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-SLAMF6 antibody produced in mouse, Monoclonal Anti-Activating NK receptor, Monoclonal Anti-CD352, Monoclonal Anti-KALI NTB-A, Monoclonal Anti-KALIb, Monoclonal Anti-Ly108, Monoclonal Anti-NK-T-B-antigen, Monoclonal Anti-NTBA, Monoclonal Anti-NTBA receptor, Monoclonal Anti-SF2000, Monoclonal Anti-SLAM family member 6, Monoclonal Anti-natural killer-, T- and B-cell antigen
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: SF24, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~43 kDa
• species reactivity: human
• concentration: ~1 mg/mL
• technique(s): flow cytometry: 10-20 μg/test using MCF7 cells.; indirect immunofluorescence: 10-20 μg/mL using MCF7 cells.
• UniProt accession no.: Q96DU3
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SLAMF6(114836)

Description

General description

Monoclonal Anti-SLAMF6 (mouse IgM isotype) is derived from the hybridoma SF24 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Signaling lymphocyte activation molecule family member 6 (SLAMF6) belongs to CD2 receptor subfamily of immunoglobulin (Ig) superfamily. It is a type I transmembrane glycoprotein. SLAMF6 consists of two extracellular immunoglobulin (Ig)-like domains and three cytoplasmic tyrosine-based signaling motifs. It is present on the surface of immune cells such as B-cells, T-cells and natural killer (NK) cells.

Specificity

Monoclonal Anti-SLAMF6 recognizes human SLAMF6.

Immunogen

synthetic peptide corresponding to an internal sequence of human SLAMF6. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

Application

Monoclonal Anti-SLAMF6 antibody produced in mouse may be used in:

• immunoblotting
• flow cytometry
• immunocytochemistry

Biochem/physiol Actions

Signaling lymphocyte activation molecule family member 6 (SLAMF6) takes part in the uncoupling of SLAM receptors from Src homology 2 domain-containing protein tyrosine phosphatase 1 (SHP-1). Overexpression of SLAMF6 is observed in B-lymphocytes from chronic lymphocytic leukemia (CLL) and lymphoma patients.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable