SAB4200542

Anti-ADAR1 (C-terminal) antibody produced in rabbit

IgG fraction of antiserum

• Synonym(s): Anti-136 kDa double-stranded RNA-binding protein, Anti-DRADA, Anti-DSH, Anti-DSRAD, Anti-G1P1, Anti-IFI-4, Anti-IFI4, Anti-K88DSRBP, Anti-P136, Anti-RNA-specific, Anti-adenosine deaminase, Anti-adenosine deaminase acting on RNA 1-A, Anti-double-stranded RNA-specific adenosine deaminase, Anti-interferon-induced protein 4
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~110 kDa
• species reactivity: human
• technique(s): immunoprecipitation (IP): 5-10 μL using lysates of human HepG2 or HeLa cells; western blot: 1:500-1:1000 using whole extracts of human HEK-293T or G361 cells
• UniProt accession no.: P55265
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ADAR(103)

Description

General description

Adenosine deaminase that acts in RNA (ADAR) 1 belongs to ADAR family. It is expressed in most of the human tissues. ADAR1 has several isoforms which include the full length ADAR1 (150 kDa) and two functionally active short isoforms (80 and 110 kDa).

The ADAR1 gene is mapped on the human chromosome at 1q21.3.

Specificity

Anti-ADAR1 (C-terminal) recognizes human ADAR1.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminal region of human ADAR1, conjugated to KLH. The corresponding sequence is identical in rat, bovine, pig, dog and monkey and differs by one amino acid in mouse.

Application

Anti-ADAR1 (C-terminal) antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Adenosine deaminase that acts in RNA (ADAR) facilitates RNA editing by catalyzing the hydrolytic deamination of adenosine (A) to inosine (I) in double-stranded (ds). ADAR1 plays critical roles in differentiating cells of embryo and adult tissues, facilitating cell survival and permitting their further differentiation and maturation. Its role in embryonic development is especially demonstrated within the hematopoietic lineage as well as in adult hematopoietic progenitor cells (HPCs). In addition, ADAR1 regulates hematopoietic stem cell maintenance and suppresses interferon signaling that may protect organisms from the harmful effects of interferon activation leading to various pathological conditions, such as chronic inflammation, autoimmune disorders, and cancer.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable